RESUMO
Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) represents a powerful tool for the analysis of biomolecules, synthetic polymers, and even small organic compounds; its performances largely depend on the type of matrix materials utilized. Here, for the first time the employment of nanoporous carbons derived from metal-organic frameworks (MOFs) as novel matrices for SALDI-MS is demonstrated. The nanoporous carbons derived from MOFs not only circumvent the shortcomings of existing matrix materials but also demonstrate much higher efficiency of laser desorption/ionization for various compounds than any other nanoporous carbons reported so far. A new perspective for the development of matrix materials for SALDI-MS application is therefore provided.
Assuntos
Carbono/química , Metais/química , Nanoporos , Compostos Orgânicos/química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Adsorção , Interações Hidrofóbicas e Hidrofílicas , Nanoporos/ultraestrutura , Espectroscopia Fotoeletrônica , Análise Espectral Raman , Vapor , Temperatura , Difração de Raios XRESUMO
A 3D metal-organic framework (MOF) nanomaterial as matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and tandem mass spectrometry (MS/MS) was developed for the analysis of complex biomolecules. Unlike other nanoparticle matrices, this MOF nanomaterial does not need chemical modification prior to use. An exceptional signal reproducibility as well as very low background interferences in analyzing mono-/di-saccharides, peptides and complex starch digests demonstrate its high potential for biomolecule assays, especially for small molecules.
